Encapsulated NOLA™ Fit 5500 Lactase—An Economically Beneficial Way to Obtain Lactose-Free Milk at Low Temperature

The current requirements of industrial biocatalysis are related to economically beneficial and environmentally friendly processes. Such a strategy engages low-temperature reactions. presented approach is essential, especially in food processes, where temperature affects the quality nutritional value foodstuffs. subject study hydrolysis lactose with commercial lactase NOLA™ Fit 5500 (NOLA). complete decomposition into two monosaccharides gives sweeter product, recommended for intolerant people those controlling product’s caloric content. reaction was performed at 15 °C, which milk transportation storage temperature. enzyme showed activity over entire range substrate concentrations (up 55 g/L lactose). For reusability easy isolation, encapsulated sodium alginate network. Its stability allows carrying out six cycles monosaccharides, lasting from four hours. During study, kinetic description native NOLA conducted. As result, model competitive galactose inhibition glucose mixed influence (competitive activation) proposed. capsule size does not rate; thus, diffusion capsules can be omitted process description. prepared 4 mm separate between cycles, e.g., using sieves.